Principles of Enzyme Catalysis
Product Code : LSK-BL-10178
Principles of Enzyme Catalysis (SKU LSK-BL-10178) is a practical kit in which students run a catalase assay on 1.2% hydrogen peroxide and calculate the rate of the enzymatic reaction from colour readings on a visible spectrophotometer. It fits Class 12 biology, B.Sc. biochemistry and biotechnology labs that teach enzyme kinetics. Science Lab Supplies in Ambala, India, offers it on quotation to colleges, schools, tender contractors and distributors, together with the pipettes and glassware the method calls for.
Specifications
The catalase kit is broken down item by item in the table. In short, it combines catalase solution, 1.2% hydrogen peroxide and phosphate buffer with an assay reagent, acidification solution, colour enhancer and colour developer, replacing the older sulfuric acid and permanganate titration with a colour-based reading.
| Specification | Value |
|---|---|
| SKU | LSK-BL-10178 |
| Experiment | Enzyme assay and rate of the enzymatic reaction |
| Topics | Enzyme catalysis, nature of enzyme action, protein structure–function relationships |
| Reagent system | No sulfuric acid or potassium permanganate needed |
| Instructions | Included |
| Enzyme | Catalase solution |
| Substrate | 1.2% hydrogen peroxide |
| Buffer | Phosphate buffer |
| Assay reagent | Included |
| Acidification solution | Included |
| Colour enhancer | Included |
| Colour developer | Included |
| Also needed (not included) | Visible wavelength spectrophotometer, pipet pumps, test tubes and racks, 1 ml, 5 ml and 10 ml pipets, beakers, graph paper, distilled water, ice |
| Supplied by | Science Lab Supplies, Ambala, India |
Applications and Experiments for Principles of Enzyme Catalysis
The kit is used to follow how much hydrogen peroxide catalase breaks down over time. Students stop the reaction at set intervals with the acidification solution, develop colour from the peroxide left, read it on a visible spectrophotometer and plot a rate curve, a quantitative step beyond bubble-counting demonstrations.
- Class 12 biology and B.Sc. biochemistry: enzyme kinetics, initial rate and the shape of a progress curve.
- Spectrophotometry practice: preparing timed samples in tubes held in a Stand for Cylinders (Test Tube Rack) and reading absorbance.
- Investigating enzyme concentration or temperature, with samples chilled on ice in a Chemical Glasses (Beaker) 250ml.
- B.Tech biotechnology: an introduction to assay design before work with purified enzymes.
Standard Scope of Supply
The kit contains instructions, catalase solution, 1.2% hydrogen peroxide, phosphate buffer, assay reagent, acidification solution, colour enhancer and colour developer. The spectrophotometer, pipet pumps, pipets, test tubes, racks, beakers, graph paper, distilled water and ice are not included. Shelf life depends on the chemical, its grade and storage conditions; ask us for the expiry date of the batch you are quoted.
Packing, MOQ and Lead Time
One kit or a multi-kit order for a biochemistry department can be quoted on request. Science Lab Supplies puts the packing, delivery time and price for that quantity on the quotation, so the figures match the order exactly, and graduated pipettes or racks can share the same quote.
As the holder of an Importer-Exporter Code, Science Lab Supplies can ship to buyers in South Africa, Qatar, Sri Lanka, Vietnam, Malaysia and Peru. A short message on the contact page or a list built in the enquiry cart starts a quote, and the lab tender page covers tender submissions.
Frequently Asked Questions
Which enzyme does Principles of Enzyme Catalysis use?
It uses catalase solution acting on 1.2% hydrogen peroxide. The remaining peroxide is turned into a colour with the developer and enhancer, so the reaction can be measured on a visible wavelength spectrophotometer.
How is it different from the Enzyme Activity Kit?
This kit uses catalase with an acidification step and colour developer. The Enzyme Activity Kit uses an extract of turnip root with guaiacol and four phosphate buffers from pH 3 to pH 14, for 10 lab groups.
Is the kit safe for student use?
It avoids sulfuric acid and potassium permanganate, but hydrogen peroxide and the acidification solution can still irritate skin and eyes. Students should wear goggles and work under teacher supervision.
Who supplies Principles of Enzyme Catalysis?
The kit is quoted and shipped by Science Lab Supplies from its base in Ambala, India, for colleges, schools and tender contractors.
Related Enzyme Practical Kits and Supplies
These Biology Lab kits teach enzyme action with other enzymes and substrates, while the glassware and reagent below support the measuring steps, so a full enzyme module can be quoted with this catalase kit.
- Enzyme Activity Kit — turnip-root assay with guaiacol and buffers at pH 3, 7, 10 and 14.
- Basic Enzymology Kit — invertase experiments on substrate specificity and inhibition, with sample graphs.
- Bacterial Amylase Project Pack — teaching notes with enzyme, starch and iodine for amylase work.
- Forensics Enzymology — saliva amylase levels used to find the sleep-deprived driver.
- Hydrogen Peroxide 6% Analytical Solution — 6% solution for oxidation and oxygen-evolution experiments.
- Graduated Pipettes 10ml — clear, graduated glass pipette with 10 ml capacity.
Browse the full Biology Lab range, the Lab Chemicals range for reagents, or all biology lab equipment.
Last updated: 9 October 2026
