Ion Exchange Chromatography Kit
Product Code : LSK-BL-10183
Ion Exchange Chromatography Kit (SKU LSK-BL-10183) shows students how a mixture of two chemicals binds to an ion-exchange column and separates during elution when the conditions change their net charge. It gives B.Sc. biochemistry, biotechnology and chemistry students, and advanced Class 12 learners, a hands-on introduction to column chromatography, with enough material for 6 separations. Science Lab Supplies in Ambala, India, can include it in quotations for colleges, schools, contractors and distributors.
Specifications
The ion-exchange kit is described component by component in the table. In short, it provides an ion exchanger, a two-chemical mixture, potassium acetate buffer and chromatography columns with instructions, for 6 separations, with the practical requiring 60–90 minutes, built on the fact that most molecules carry a net charge between pH 2 and 10.
| Specification | Value |
|---|---|
| SKU | LSK-BL-10183 |
| Technique | Ion-exchange column chromatography |
| Sample | Mixture of two chemicals |
| Principle | Separation during elution under conditions that change net charge |
| Charge range | Most molecules have a net charge within pH 2 to 10 |
| Instructions | Included |
| Ion exchanger | Included |
| Chemical mixture | Included |
| Buffer | Potassium acetate buffer |
| Columns | Chromatography columns |
| Capacity | 6 separations |
| Time required | 60–90 minutes |
| Supplied by | Science Lab Supplies, Ambala, India |
Applications and Experiments for the Ion Exchange Chromatography Kit
The kit is used to demonstrate charge-based separation, the method behind protein and amino acid purification. Students load the two-chemical mixture onto the ion exchanger, elute it with buffer, and see the components leave the column at different stages once the pH shifts the net charge of each molecule.
- B.Sc. biochemistry and biotechnology: principles of ion-exchange chromatography and protein purification.
- Class 12 chemistry and biology: how pH affects the charge of molecules, using buffer volumes measured in a Measuring Cylinder Glass.
- Collecting eluted fractions in tubes held upright in a Test Tube Rack SS and comparing them.
- Comparing column methods with paper chromatography as part of a separation-techniques unit.
Standard Scope of Supply
The kit contains instructions, an ion exchanger, a chemical mixture, potassium acetate buffer and chromatography columns, enough for 6 separations. Fraction tubes, racks, measuring glassware and any column stands are not included and can be quoted with the kit. Shelf life depends on the chemical, its grade and storage conditions; ask us for the expiry date of the batch you are quoted.
Packing, MOQ and Lead Time
Prices can be quoted for one kit or for several kits when more groups need to run separations at the same time. The Science Lab Supplies quotation shows the packing, delivery time and price for that number, and test tubes or measuring cylinders can be added to it.
Science Lab Supplies is a registered exporter holding an Importer-Exporter Code, so it can ship to buyers in Peru, Egypt, Iraq, Nigeria, Kenya and Ghana. Start a quote from the contact page or the enquiry cart; for formal tenders, the lab tender page explains the process.
Frequently Asked Questions
How many separations does one Ion Exchange Chromatography Kit cover?
One kit covers 6 separations. The practical requires 60 to 90 minutes, so six groups can each run one separation within a single long session.
Why does pH matter in ion-exchange chromatography?
Most molecules carry a net charge between pH 2 and 10, and changing the pH can change that charge sharply. A molecule binds to or leaves the ion exchanger according to its charge, so pH controls the separation.
What else is needed to run the kit?
The kit provides the ion exchanger, chemical mixture, buffer and columns. Laboratories provide tubes or vessels to collect fractions, a rack and basic measuring glassware, which can be quoted on the same order.
Who supplies this chromatography kit?
Science Lab Supplies ships it from Ambala, India, on quotation to colleges, schools, distributors and tender contractors, together with any glassware requested.
Related Separation and Analysis Items
These chromatography and electrophoresis items from the Biology Lab and Chemistry Lab ranges show other ways of separating mixtures, from paper strips to HPLC and gels, so a complete separation-techniques unit can be priced together.
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- Chromatographic Paper — 110 x 140 mm laboratory grade paper for crisp pigment separation.
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- Electrophoresis Buffer, 10X TBE — Tris-borate EDTA buffer for electrophoresis, enough for 5 litres.
- Blue Light Transilluminator — views stained DNA gels without UV over a 7 x 14 cm area.
- Enzyme Activity Kit — enzyme assay comparing phosphate buffers at pH 3, 7, 10 and 14.
- Test Tubes — pack of 100 tubes for holding and observing small volumes.
Browse the full Biology Lab range, the Chemistry Lab range for chromatography supplies, or all biology lab equipment.
Last updated: 9 October 2026
